![]() ![]() Compared with molecular weight markers, Southern blotting can confirm the identity, size, and abundance of hybridized probe targets. The general Southern blotting method is to transfer the sample DNA to a suitable membrane after restriction endonuclease digestion and gel electrophoresis, and use labeled specific nucleic acid fragments as probes for detection. Steps in the procedure for transferring DNA from an agarose gel strip to a hybridization membrane. We provide comprehensive nucleic acid blotting services to analyze genes and their expression in cell banks.įig 1. Regulatory authorities require confirmation that the genomic DNA structure of the cell bank is stable and unchanged and that there is no clonal rearrangement. Recombinant expression cell lines are commonly used in modern laboratory research and production of biologically active peptides, especially when used as human or animal drugs or biological preparations. ![]() These methods can provide critical information about gene expression and genome structure. Southern blotting (Southern) and Northern blotting (Northern) permanently bind nucleic acids to these membranes and hybridize with specific nucleic acid probes. Nucleic acids are usually transferred from agarose gels to membrane supports (such as nitrocellulose or coated nylon membranes) by capillary action. Common blots in modern laboratories include Northern blots, Southern blots, and dot/slot blots. The transfer of nucleic acids or proteins to the microporous membrane is called "blotting", and the term includes "spotting" (sample deposition) and transfers from a flat gel. We have established mature solutions that can provide a fast and comprehensive analysis. This service can help researchers determine the plasmid copy number and restriction enzyme fragment analysis, genetic monitoring, mapping, and gene expression analysis in transfected cells.
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